52nd Symposium of the Society for Histochemistry

52^nd Symposium of the Society for Histochemistry Prague, 1 - 4 September, 2010

Advanced imaging techniques in biomedicine:
from molecules to organisms

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FINAL SCIENTIFIC PROGRAMME

Organized in collaboration with

	Wednesday, 1 September
14:00	Registration desk open
17:30 - 18:30	Welcome drink and light refreshment
18:30 - 19:00	Symposium opening, awarding the Robert Feulgen Prize and young histochemist awards
19:00 - 19:45	The Robert Feulgen Lecture (Chair: Pavel Hozák) Stefan W. Hell: Nanoscopy with focused light

	Thursday, 2 September
9:00 - 10:10	Workshop I: High-resolution light microscopy methods (Chair: Lucie Kubínová) 9:00 - 9:35 Christoph Cremer (invited): Nanoimaging of cellular structure by spectrally assigned localization and structured illumination microscopy 9:35 - 10:10 Heinrich Leonhardt (invited): Studying nuclear structure and function with 3D structured illumination microscopy (3D-SIM) and fluorescent nanobodies
10:10 - 10:55	Carl Zeiss workshop Joerg Lindenau: Next generation in LSM detector technology in combination with superresolution - the LSM 780 Elyra PS1
10:55 - 11:25	Coffee break
11:25 - 12:10	Leica Microsystems workshop Susanne Liebe: Leica TCS STED CW: The fast track to Super-resolution
12:25 - 13:10	Applied Precision lunch workshop Jürgen Neumann: Putting cells behind bars - principles and applications of Structured llumination Microscopy
13:10 - 14:15	Workshop II: Imaging of molecules and events in living systems (Chair: Jaromír Plášek) 13:10 - 13:45 Martin Hof (invited): Recent developments in fluorescence correlation spectroscopy 13:45 - 14:00 Cornelis J. Van Noorden: Controlled light exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging 14:00 - 14:15 David Staněk: The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells
14:15 - 15:10	Poster session with a coffee break (Part I - odd numbers of posters)
15:10 - 17:30	Workshop III: Microscopy in diagnostics and clinical treatment (Chair: Cornelis J. Van Noorden) 15:10 - 15:35 Eva Syková (invited): MR imaging of stem cells in vivo 15:35 - 16:10 Davor Ježek (invited): The role of confocal and electron microscopy in visualization of biocrystals in clinical specimens 16:10 - 16:45 Daniel Razansky (invited): Virtual sectioning of intact and living tissues using advanced optical and optoacoustic tomography methods 16:45 - 17:00 Lucie Kubínová: The error in measurement of capillary supply of skeletal muscle fibres from thin cross sections and its reduction by 3D method using confocal microscopy 17:00 - 17:15 Josef Makovitzky: Amyloid - origin, history and submicroscopical structure 17:15 - 17:30 Guenter Schmidt: Identification of prognostic factors using quantitative image analysis of HER2 expression by immunohistochemistry (IHC) in adenocarcinoma of the oesophagogastric junction
17:30 - 19:10	Workshop IV: Novel approaches to study histological samples (Chair: Juergen Roth) 17:30 - 18:05 Axel Walch (invited): MALDI imaging mass spectrometry for direct tissue analysis: Current state and fields of application in molecular histology 18:05 - 18:40 Michael Volný (invited): Mass spectrometry imaging in lipid analysis of histological sections 18:40 - 18:55 Cornelis J. Van Noorden: Imaging enzymes at work: Metabolic mapping by enzyme histochemistry 18:55 - 19:10 David Větvička: Whole body imaging - magnificient tool for (not only) drug delivery research
	Friday, 3 September
9:00 - 10:20	Workshop V: Advances in sample preparation and detection of targets in electron microscopy (Chair: Jana Nebesářová) 9:00 - 9:35 Ben Giepmans (invited): New probes and imaging methods for correlated light and electron microscopy 9:35 - 9:50 Pavel Hozák: Developing new tools for simultaneous detection of multiple molecular targets 9:50 - 10:05 Margarita Sobol: Improvements of sample processing for sensitive ultrastructural immunogold labeling 10:05 - 10:20 Igor Kireev: In-vivo immunolabeling for electron microscopy bymicroinjection of Nanogold-coupled antibodies
10:20 - 10:40	Carl Zeiss workshop Peter Gnauck: High resolution FIB-nanotomography of bilogical cell tissue and investigation of interfaces between tissue and biocompatible materials
10:40 - 11:10	Coffee break
11:10 - 12:15	Workshop VI: Advanced imaging techniques in life sciences and medicine (Chair: Marco Biggiogera) 11:10 - 11:45 Nadejda B. Matsko (invited): Biocalcification. Correlative AFM and EM microscopy and spectroscopy 11:45 - 12:00 Jana Nebesářová: Detection of metal nanoparticles by means of FE SEM 12:00 -12:15 Evelyn A. Wallnoefer: MRI Molecular Imaging with nanoparticles: first results with a vascular model
12:30 - 13:15	FEI lunch workshop Wim F. Voorhout: Ultrastructural cell architecture in 3D: new developments in 3D electron microscopy and correlative workflows
13:15 - 14:15	Poster session (Part II - even numbers of posters)
14:15 - 15:00	Leica Microsystems workshop Udo Graf: Versatile transfer system for cryo SEM
15:00 - 16:05	Workshop VII: Cryoelectron and correlative light-electron microscopy (Chair: Margit Pavelka) 15:00 - 15:35 Judith Klumperman (invited): Imaging the endo-lysosomal system by correlative live cell – electron microscopy 15:35 - 15:50 Clemens Röhrl: Differentiated cellular pathways of lipids visualized by combined light and electron microscopy using DAB-photooxidation 15:50 - 16:05 Claudia Meisslitzer-Ruppitsch: The ceramide-enriched trans-Golgi compartments reorganize in response to ATP-depletion
16:05 - 17:05	Business meeting of the Society for Histochemistry Agenda: - President´s report, Secretary´s report, Treasure´s report - Report on Histochemistry and Cell Biology Journal - Next Symposium - Miscellaneous
18:30	Transportation to the banquet site
19:00	Symposium banquet

	Saturday, 4 September
9:30 - 10:00	Robert Feulgen Prize 2010 laureate lecture (Chair: Pavel Hozák) Heidi de Wit: Ultrastructural analysis of the minimal docking machinery for secretory vesicles
10:00 - 12:00	Workshop VIII: Free topics communications (Chair: Paul Debbage and Ioannis Mylonas) 10:00 - 10:15 Igor Buchwalow: Keratin 5/14+ progenitor cell concept of breast epithelium: Revisited 10:15 - 10:30 Christian Schöfer: Chromosomal bar-coding by epigenetic memory effect of active chromatin marker histone variant H3.3 10:30 - 10:45 Masahiko Harata: Roles of actin-related proteins in nuclear organization and genome function 10:45 - 11:00 Daniela Buckiová: Transport of nanoparticles from the middle ear to the cochlea 11:00 - 11:15 Marco Biggiogera: Nuclear body-mediated transport of RNAse 11:15 - 11:30 Samo Hudoklin: Imaging the formation of urothelial plaques in urinary bladder 11:30 - 11:45 Sukriye Yildirim: Roles of complexes of nuclear myosin 1 and lipids in the cell nucleus
12:00 - 12:15	Closing of the symposium + poster awards

List of Posters

P-01	Irina B.	Alieva	HPAEC in vitro is a physiologically-relevant cellular model to study the involvement of microtubules in the barrier-protective/compromising mechanisms
P-02	Marlene	Benchimol	Cell viability tests to track the effects of Tritrichomonas foetus and Trichomonas vaginalis on oviduct cells cultures
P-03	Manuel	Bobrich	PTPIP51 – a new marker for ependymoma?
P-04	Ansgar	Brüning	Analysis of the endoplasmic reticulum stress response in human cancer cells
P-05	Igor	Buchwalow	Non-specific immunostaining of endogenous Fc receptors: Demystified
P-06	Vitalija	Buneviciene	Expression of matrix metalloproteinase-3 and -9 in human ischemic myocardium
P-07	Martin	Čapek	Volume visualization of a rat embryo by using confocal microscopy and optical projection microscopy
P-08	Jiří	Dammer	Micro-radiography as Supporting Technique for Imaging of Biological Samples prepared for EM
P-09	Hana	Demova	Chromosomal damage in peripheral blood lymphocytes of newly diagnosed cancer patients and healthy controls
P-10	Lyubov	Didenko	Ultrastructural diagnostic of microorganisms in biological material during infectious-inflammatory processes
P-11	Silvia	Fittipaldi	C-Reactive Protein levels can predict embolization risk during Carotid Artery Stenting.
P-12	Aleš	Folvarčný	Innovative Research of Electron Microscopes, Optimization Possibilities of Modern Types Magnetic Lens and Chambers - part 1: Magnetic Properties of Steel for Magnetic Lens and Chambers
P-13	Regina	Holčáková	Innovative Research of Electron Microscopes, Optimization Possibilities of Modern Types Magnetic Lens and Chambers - part 2: Simulation of Layout Magnetic Field of Types Magnetic Lens
P-14	Samo	Hudoklin	Jacalin binding and apical plasma membrane structure in low grade papillary urothelial tumors induced by N-butyl-N-(4-hidroxybutyl)nitrosamine in rats
P-15	Aneta	Jebáčková	Methodology of Dictyostelium discoideum imaging with the transmission digital holographic microscope
P-16	Peter	Jurisch	PTPIP51- a possible molecule to understand colorectal cancer
P-17	Murray C.	Killingsworth	Immunoelectron microscopy using routinely processed pathology tissue
P-18	Jan	Klepetář	Evaluation of Confocal and Two-Photon Microscopy performance:Application of stabile Fluorophore Films and Layers
P-19	Aleksandra	Korac	Mitochondrial distribution and nitric oxide production in partially fragmented human embryo
P-20	Elena A.	Kost	The research of fibroblast McCoy cell culture, infected by Chlamydia trachomatis
P-21	G.	Krasteva	Tracheal brush cells are neuronally connected cholinergic sensory cells
P-22	Josef	Makovitzky	Cellular internalization of nanoparticles in culture systems: A polarization microscopical study
P-23	Christina	Matsingou	Expression and regulation of inhibins in gynaecological cancer cells
P-24	Stanislava	Merjava	A combination of indirect fluorescent immunohistochemistry with fluorescent in situ hybridization in a patient with posterior polymorphous corneal dystrophy
P-25	Jan	Michálek	Automatic correction of lateral brightness variation in large stacks of confocal laser scanning microscope images.
P-26	Aimar	Namm	Immunohistochemical detection of BMP-2 and BMP-4 in early stages of neural tube formation in human embryos
P-27	Dalia	Pangonyte	Left ventricular fibrillar collagenous matrix remodelling in chronic ischemia
P-28	Tamara	Pecenkova	The role of the exocyst complex in the innate immunity of plants
P-29	Radek	Pelc	Plant specimens as testing objects for microscopic optical contrasting
P-30	Zdeněk	Pilát	Spectroscopy and Optical Trapping of Oil-Rich Algal Cells in Microfluidic Systems for in vivo Analysis and Sorting
P-31	Barbora	Radochová	Effects of chemical fixation and freeze substitution on preservation of chloroplast ultrastructure of Fagus sylvatica leaves and Picea abies needles
P-32	Jana	Rohožková	“Cytoskeletal” proteins in nucleus – nucleoplasmic shuttling of vinculin and actinin
P-33	Natalia	Shevlyagina	Correlative microscopy for the detection of spores
P-34	Christian	Schöfer	Ultrastructural detection of nuclear antigens using polymer amplification and enzyme metallography
P-35	Evangelia	Skarmoutsou	Expression and localization of prominin-1 in rat parotid gland after stimulation with isoproterenol
P-36	Mitsuo	Suga	The New Atmospheric Scanning Electron Microscope observes cells in solution as an optical- and electron-correlative microscope
P-37	Kanae	Teramoto	Observation of Cells with a New Atmospheric Scanning Electron Microscope
P-38	Gudrun C.	Thurner	Albumin nanoparticles for use in MRI: essential requirements for molecular imaging molecules
P-39	Vlastimil	Tichý	Study of local open DNA structures and their interaction with p53 proteins by Atomic Force Microscopy
P-40	Johana	Trojanová	The subcellular distribution of presynaptic glycine receptors correlates with the occurrence of endogenous sources of their agonists
P-41	Marie	Vancová	Application of new metal nanoparticles for multiple labelling in electron microscopy
P-42	Naděžda	Vaškovicová	Differences in the structure of the plasma membrane HL-60 cells
P-43	Tomáš	Venit	Determination of tissue and cell function of Nuclear Myosin 1 by gene knock out
P-44	Zdeňka	Zemanová	Proliferation of tubular and glomerular epithelia in the chick embryonic kidney depends on the nephron position and age

instructions for poster presentation

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